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Links for Methods:

  • Acid-Fast Stain Protocols

    The Ziehl-Neelsen method has endured as a reliable and effective way to demonstrate the acid-fast bacteria. By ASM MicrobeLibrary.

    http://www.microbelibrary.org/component/resource/laboratory-test/2870-acid-fast-stain-protocols
  • Agar Overlay Technique

    This technique allows you to produce a homogeneous lawn of bacteria within a thin layer of agar across the surface of a plate. By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1994/05/04/agar-overlay-technique/
  • Assay for Coliform Contamination in Ambient Water

    The presence of coliform bacteria is widely used as an indicator of potential fecal contamination. By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1993/07/27/assay-for-coliform-contamination-in-ambient-water/
  • Autoclave Use

    Heating is one of the most convenient methods for sterilization. By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1985/10/15/autoclave-use/
  • Bacterial Contamination of Meat: Pour Plate Assay

    By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1989/07/05/bacterial-contamination-of-meat-pour-plate-assay/
  • Bacterial Contamination of Milk: Pour Plate Assay

    Fresh food will typically have very low bacterial content, but as it is handled and stored, the bacterial concentration may increase dramatically. By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1989/07/05/bacterial-contamination-of-milk-pour-plate-assay/
  • Bacterial Growth Curve: E coli on various types of liquid media

    Bacteria display a characteristic four-phase pattern of growth in liquid culture. By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1992/08/03/bacterial-growth-curve/
  • Bacteriological Analytical Manual (BAM)

    U.S. Food & Drug Administration's Bacteriological Analytical Manual (BAM) presents the agency's preferred laboratory procedures for microbiological analyses of foods and cosmetics.

    http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm2006949.htm
  • Bacteriological Smear and Staining Protocol

    Bacteria must be stained in order to be easily visualized under the microscope. By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1980/02/11/bacteriological-smear-and-staining-protocol/
  • Biological Procedures Online

    An international peer-reviewed scientific journal serving the medical and biological research community with research methods and protocols.

    http://www.biologicalproceduresonline.com/
  • Bruce A. Roe's Molecular Methods

    Protocols used in Bruce Roe's Laboratory.

    http://www.genome.ou.edu/proto.html
  • Buccal Smear

    By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/2001/07/11/buccal-smear/
  • Coliform in Drinking Water Assay

    The health standard for drinking water allows no more than 5 coliform per hundred mL. By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1989/07/20/coliform-in-drinking-water/
  • Commonly Used Media for Phage Growth

    By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1990/06/24/commonly-used-media-for-phage-growth/
  • Commonly Used Solid Media and Other Common Microbiological Media

    These media are used for a variety applications in Microbiology. By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1990/06/24/commonly-used-solid-media/
  • Culture Purification

    Testing pure cultures is essential to the success of the any microbial identification system. By Arrow Scientific Pty Ltd.

    http://www.arrowscientific.com.au/index.php?option=com_content&view=article&id=47%3Aculture-purification&catid=25&Itemid=40
  • Cyanosite: Cyanobacterial protocols

    Containing links to protocols useful for cyanobacterial research.

    http://www-cyanosite.bio.purdue.edu/protocols/protocols.html
  • Differentiating Gram-negative and Gram-positive Bacteria

    The Gram-Stain is the single most common and cost effective staining technique used in identifying bacterial organisms. By Arrow Scientific Pty Ltd.

    http://www.arrowscientific.com.au/index.php?option=com_content&view=article&id=48%3Adifferentiating-gram-negative-and-gram-positive-bacteria&catid=25&Itemid=40
  • Dilutions: Principles and Applications

    It is frequently necessary to dilute these solutions to a desired level. By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1993/07/02/dilutions-principles-and-apllications/
  • Displacement Pipetters: Their Care and Use

    By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/2001/07/18/displacement-pipetters-their-care-and-use/
  • Enumeration of PAH-degraders in soil

    By The National Environmental Research Institute in Denmark.

    http://www2.dmu.dk/1_Viden/2_Miljoe-tilstand/3_jord/4_microtitermethods/
  • Environmental microbiological sampling using aseptic methods

    While sampling alone cannot guarantee the safety of your product, it should be an integral part of your food safety system. By Arrow Scientific Pty Ltd.

    http://www.arrowscientific.com.au/index.php?option=com_content&view=article&id=49%3Aenvironmental-microbiological-sampling-using-aseptic-methods&catid=25&Itemid=40
  • EPA Microbiology Home Page

    EPA methods related to bacteria, viruses and protozoans can be found at this site.

    http://www.epa.gov/nerlcwww/
  • Fluorescent In Situ Hybridization: (FISH)

    FISH is a molecular technique that is often used to identify and enumerate specific microbial groups. By Microbial Life - Educational Resources.

    http://serc.carleton.edu/microbelife/research_methods/microscopy/fish.html
  • Gram Stain Protocol - Hucker's Modification

    By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1993/07/14/gram-stain-protocol/
  • Hemolytic Streptococcus Detection by Throat Culture

    By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1993/07/27/hemolytic-streptoccus-detection/
  • Measuring pH in meat and meat products

    Meat quality is greatly influenced by muscle pH and its measurement is important. By Arrow Scientific Pty Ltd.

    http://www.arrowscientific.com.au/index.php?option=com_content&view=article&id=59%3Ameasuring-ph-in-meat-and-meat-products&catid=17&Itemid=31
  • Meat and Milk Assay Setup

    By Dr. David B. Fankhauser, U.C. Clermont College.

    http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Meat_Milk/meat&milk_setup.htm
  • Microbiological Media Preparation

    By Dr. David B. Fankhauser, U.C. Clermont College.

    http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Media_Prep/Media_Prep.htm
  • Plasmids and Protocols

    By The Yeast Resource Center (YRC). A Biomedical Technology Research Center supported by the National Institute of General Medical Studies at the US National Institutes of Health.

    http://depts.washington.edu/yeastrc/
  • Pour Plate Technique for Bacterial Enumeration

    The pour plate technique can be used to determine the number of microbes/mL or microbes/gram in a specimen. By Dr. David B. Fankhauser, U.C. Clermont College.

    http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Meat_Milk/Pour_Plate.htm
  • Preparation of Bacteriophage Stocks

    By Dr. David B. Fankhauser, U.C. Clermont College.

    http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Phage/Phage_Prep.htm
  • Preparation of Wet Mount Slide

    Wet mount preparations are especially valuable for demonstrating motility in microorganisms. By Dr. David B. Fankhauser, U.C. Clermont College.

    http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Wet_Mount/Wet_Mount.htm
  • Sample Math Problems for Microbiology

    Calculations for calculating microbe per unit volume. By Dr. David B. Fankhauser, U.C. Clermont College.

    http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Micro_Math_Problems.htm
  • Serial Dilution, Pipetting Practice

    Serial dilutions are regularly used in microbiology when initial concentrations of bacteria are much too high to perform a plate count, or for producing a series of regular dilutions as in titering serum. By Dr. David B. Fankhauser, U.C. Clermont College.

    http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Yeast_Plate_Count/Serial_Dilutions.htm
  • Single Colony Isolation

    By Dr. David B. Fankhauser, U.C. Clermont College.

    http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Coliform_assays/Single_Colony_Isolation.htm
  • Spectrophotometer Use

    The spectrophotometer is an instrument which measures the amount of light of a specificed wavelength which passes through a medium. By Dr. David B. Fankhauser, U.C. Clermont College.

    http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Growth_Curve/Spectrophotometer.htm
  • Spreading Technique for Plating Bacteria

    By Dr. David B. Fankhauser, U.C. Clermont College.

    http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Yeast_Plate_Count/Spreading_Technique.html
  • Sterile Technique: Delivery of Liquids by Pipet

    By Dr. David B. Fankhauser, U.C. Clermont College.

    http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Yeast_Plate_Count/Sterile_Tech_pipet.htm
  • Summary of Biochemical Tests

    By Rachel Watson, M.S. at the University of Wyoming.

    http://www.uwyo.edu/molb2210_lab/info/biochemical_tests.htm
  • The Australian based Antibiotic Susceptibility Testing group

    This site is maintained as an on-line resource for laboratory scientists performing antibiotic susceptibility testing.

    http://web.med.unsw.edu.au/cdstest/
  • The Gottschling Lab

    Dan Gottschling's laboratory at the Fred Hutchinson Cancer Research Center in Seattle with a list of useful protocols used for studying microbes.

    http://research.fhcrc.org/gottschling/en.html
  • The Gram Stain

    Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents. By Microbial Life - Educational Resources.

    http://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html
  • Thermometers - calibrating them

    To calibrate a thermometer only takes a few minutes and is essential for good business practices. By Arrow Scientific Pty Ltd.

    http://www.arrowscientific.com.au/index.php?option=com_content&view=article&id=66%3Athermometers-calibrating-them&catid=17&Itemid=31
  • Thermometers - using them

    The correct use of a thermometer is one of the most important step in undertaking HACCP monitoring. By Arrow Scientific Pty Ltd.

    http://www.arrowscientific.com.au/index.php?option=com_content&view=article&id=67%3Athermometers-using-them&catid=17&Itemid=31
  • Titering of Bacterial Viruses

    By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1993/02/17/titering-of-bacterial-viruses/
  • Triple Sugar Iron Agar and Its Use

    By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1987/08/03/triple-sugar-iron-agar-and-its-use-and-interpretation/
  • Yeast Plate Count Protocol

    The plate count is one of the most accurate means of enumeration of viable microbes because you get a visual indicator for every cell in the specimen. By By Dr. David B. Fankhauser, U.C. Clermont College.

    https://fankhauserblog.wordpress.com/1988/06/29/yeast-plate-count-protocol/

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